Aim: formation when isolates were treated with ursolic

Aim: The present study
was carried out to compare the sensitivity of biofilm detection methods, and to
study the effect of ursolic acid as a potential anti biofilm agent in
dispersing the slime generated by Staphylococcal sp isolated from milk
samples of crossbred dairy cows. Further, in the Staphylococcal isolates, the
presence of intracellular adherence gene locus involved in biofilm production (icaD)
was investigated and compared with the other pathogenic gene loci mecA
and pvl.

Materials and Methods:
A total of 50 S. aureus strains were isolated
over a period of 3 months from 281 milk samples collected from crossbred dairy
cows on the date of drying. These isolates were subjected for biofilm detection
by Congo Red Agar (CRA), Microtitre Plate Assay (MTP) and Polymerase Chain
Reaction specific for icaD gene.
The antagonistic effect of biofilm formation by ursolic acid was studied by
using different concentrations (30µg/ml and 60µg/ml) of ursolic acid and
compared with the control group. Molecular detection by PCR of icaD, mecA
and pvl genes
involved in biofilm production, methicillin résistance and production of
leukocidin toxin, respectively in the isolated Staphylococcus sp was also undertaken.

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Results: Current study
showed that PCR (100%) was highly sensitive, compared to MTP (90%) and CRA
(34%) methods. The in vitro studies on
effect of ursolic acid in inhibiting biofilm formation by Staphylococcus
aureus using MTP assay showed
71.5% and 48.6% inhibition at ursolic acid concentrations of 60µg/ml and
30µg/ml, respectively with a significant
difference (P